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rfp fcho2  (Addgene inc)


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    Addgene inc rfp fcho2
    Rfp Fcho2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rfp+fcho2/FCHo2-pmCherryC1+(Plasmid+%2327686)/bio_rxiv__64898__2026__03__03__709279-257-7-8
    Average 93 stars, based on 7 article reviews
    rfp fcho2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Membrane Curvature Activates Src kinase and Promotes Metastatic Cancer Cell Survival
    Article Snippet: RFP-CIP4 was a gift from Christien Merrifield (Addgene #27685). .. The FCHo2 coding sequence was amplified from RFP-FCHo2 (Addgene #27686; gift from Christien Merrifield) and subcloned into pEGFP-N1 to produce GFP-FCHo2. ..

    Amplification:

    Article Title: Membrane Curvature Activates Src kinase and Promotes Metastatic Cancer Cell Survival
    Article Snippet: RFP-CIP4 was a gift from Christien Merrifield (Addgene #27685). .. The FCHo2 coding sequence was amplified from RFP-FCHo2 (Addgene #27686; gift from Christien Merrifield) and subcloned into pEGFP-N1 to produce GFP-FCHo2. ..



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    Fig. 4 | Curved adhesions require a curvature-sensing protein, FCHo2. a, <t>RFP–FCHo2</t> positively correlates with ITGβ5–GFP in curved adhesions (arrows) at vitronectin-coated nanopillars. In the same image, FCHo2 is absent in focal adhesions (arrowheads) and clathrin-containing adhesions (thin arrows). Scale bar, 10 µm (full size) or 5 µm (insets). b, Scatter plot of RFP–FCHo2 intensity against the ITGβ5/membrane ratio at n = 353 nanopillars from the cell shown in a. See g for the correlation between ITGβ5 and FCHo2 at nanopillars, analysed in multiple cells. c, Kymographs of ITGβ5–GFP and RFP–FCHo2 at vitronectin- coated nanopillars imaged at 15 s per frame for 20 min. Scale bar, 1 µm. d, The temporal standard deviations of RFP–FCHo2 for 20 min at n = 104 high β5 and 313 low β5 nanopillars, from 2 independent cells. For each cell, nanopillars were divided into two groups based on ITGβ5 intensities: high-β5 nanopillars (top 25%) and low-β5 nanopillars (bottom 75%). Standard deviation values are normalized to the initial intensities. Medians (lines) and quartiles (dotted lines) are shown. e, FCHo2 knockdown significantly reduces nanopillar-induced ITGβ5 accumulation. BFP expression is a marker of shRNA transfection. Scale bar, 10 µm (full size) or 5 µm (insets). f, ITGβ5 does not colocalize <t>with</t> <t>FCHo1</t> at nanopillars. Scale bar, 10 µm (full size) or 5 µm (insets). g, Quantification showing that FCHo2 knockdown significantly reduces nanopillar-induced ITGβ5 accumulation,
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    Fig. 4 | Curved adhesions require a curvature-sensing protein, FCHo2. a, <t>RFP–FCHo2</t> positively correlates with ITGβ5–GFP in curved adhesions (arrows) at vitronectin-coated nanopillars. In the same image, FCHo2 is absent in focal adhesions (arrowheads) and clathrin-containing adhesions (thin arrows). Scale bar, 10 µm (full size) or 5 µm (insets). b, Scatter plot of RFP–FCHo2 intensity against the ITGβ5/membrane ratio at n = 353 nanopillars from the cell shown in a. See g for the correlation between ITGβ5 and FCHo2 at nanopillars, analysed in multiple cells. c, Kymographs of ITGβ5–GFP and RFP–FCHo2 at vitronectin- coated nanopillars imaged at 15 s per frame for 20 min. Scale bar, 1 µm. d, The temporal standard deviations of RFP–FCHo2 for 20 min at n = 104 high β5 and 313 low β5 nanopillars, from 2 independent cells. For each cell, nanopillars were divided into two groups based on ITGβ5 intensities: high-β5 nanopillars (top 25%) and low-β5 nanopillars (bottom 75%). Standard deviation values are normalized to the initial intensities. Medians (lines) and quartiles (dotted lines) are shown. e, FCHo2 knockdown significantly reduces nanopillar-induced ITGβ5 accumulation. BFP expression is a marker of shRNA transfection. Scale bar, 10 µm (full size) or 5 µm (insets). f, ITGβ5 does not colocalize <t>with</t> <t>FCHo1</t> at nanopillars. Scale bar, 10 µm (full size) or 5 µm (insets). g, Quantification showing that FCHo2 knockdown significantly reduces nanopillar-induced ITGβ5 accumulation,
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    Fig. 4 | Curved adhesions require a curvature-sensing protein, FCHo2. a, RFP–FCHo2 positively correlates with ITGβ5–GFP in curved adhesions (arrows) at vitronectin-coated nanopillars. In the same image, FCHo2 is absent in focal adhesions (arrowheads) and clathrin-containing adhesions (thin arrows). Scale bar, 10 µm (full size) or 5 µm (insets). b, Scatter plot of RFP–FCHo2 intensity against the ITGβ5/membrane ratio at n = 353 nanopillars from the cell shown in a. See g for the correlation between ITGβ5 and FCHo2 at nanopillars, analysed in multiple cells. c, Kymographs of ITGβ5–GFP and RFP–FCHo2 at vitronectin- coated nanopillars imaged at 15 s per frame for 20 min. Scale bar, 1 µm. d, The temporal standard deviations of RFP–FCHo2 for 20 min at n = 104 high β5 and 313 low β5 nanopillars, from 2 independent cells. For each cell, nanopillars were divided into two groups based on ITGβ5 intensities: high-β5 nanopillars (top 25%) and low-β5 nanopillars (bottom 75%). Standard deviation values are normalized to the initial intensities. Medians (lines) and quartiles (dotted lines) are shown. e, FCHo2 knockdown significantly reduces nanopillar-induced ITGβ5 accumulation. BFP expression is a marker of shRNA transfection. Scale bar, 10 µm (full size) or 5 µm (insets). f, ITGβ5 does not colocalize with FCHo1 at nanopillars. Scale bar, 10 µm (full size) or 5 µm (insets). g, Quantification showing that FCHo2 knockdown significantly reduces nanopillar-induced ITGβ5 accumulation,

    Journal: Nature cell biology

    Article Title: Curved adhesions mediate cell attachment to soft matrix fibres in three dimensions.

    doi: 10.1038/s41556-023-01238-1

    Figure Lengend Snippet: Fig. 4 | Curved adhesions require a curvature-sensing protein, FCHo2. a, RFP–FCHo2 positively correlates with ITGβ5–GFP in curved adhesions (arrows) at vitronectin-coated nanopillars. In the same image, FCHo2 is absent in focal adhesions (arrowheads) and clathrin-containing adhesions (thin arrows). Scale bar, 10 µm (full size) or 5 µm (insets). b, Scatter plot of RFP–FCHo2 intensity against the ITGβ5/membrane ratio at n = 353 nanopillars from the cell shown in a. See g for the correlation between ITGβ5 and FCHo2 at nanopillars, analysed in multiple cells. c, Kymographs of ITGβ5–GFP and RFP–FCHo2 at vitronectin- coated nanopillars imaged at 15 s per frame for 20 min. Scale bar, 1 µm. d, The temporal standard deviations of RFP–FCHo2 for 20 min at n = 104 high β5 and 313 low β5 nanopillars, from 2 independent cells. For each cell, nanopillars were divided into two groups based on ITGβ5 intensities: high-β5 nanopillars (top 25%) and low-β5 nanopillars (bottom 75%). Standard deviation values are normalized to the initial intensities. Medians (lines) and quartiles (dotted lines) are shown. e, FCHo2 knockdown significantly reduces nanopillar-induced ITGβ5 accumulation. BFP expression is a marker of shRNA transfection. Scale bar, 10 µm (full size) or 5 µm (insets). f, ITGβ5 does not colocalize with FCHo1 at nanopillars. Scale bar, 10 µm (full size) or 5 µm (insets). g, Quantification showing that FCHo2 knockdown significantly reduces nanopillar-induced ITGβ5 accumulation,

    Article Snippet: The expression vectors of RFP–FCHo1, RFP–FCHo2 and EPS15– RFP were gifts from C. Merrifield (Addgene, 27690, 27686 and 27696, respectively).

    Techniques: Membrane, Standard Deviation, Knockdown, Expressing, Marker, shRNA, Transfection